Reduced Glutathione (GSH) Colorimetric Assay Kit
还原型谷胱甘肽(GSH)比色法测试盒
| 规格： | / 50 assays(48 samples) / 100 assays(96 samples) |
| 价格： | / ￥200 / ￥320 |
货号：E-BC-K030-S
检测仪器及波长: 紫外-可见光分光光度计（420 nm）
样本类型: 血清;血浆;动植物组织样本及培养细胞
检测原理
还原型谷胱甘肽（GSH）可与二硫代二硝基苯甲酸（DTNB）反应产生硫代硝基苯甲酸和谷胱甘肽二硫化物（反应式见下图），硝基巯基苯甲酸是一种黄色化合物，在420 nm处，可进行比色定量测定还原型谷胱甘肽（GSH）的含量。
关键信息
| 指标简称 | GSH |
| 样本类型 | 血清;血浆;动植物组织样本及培养细胞 |
| 灵敏度 | 0.26 mg GSH/L |
| 检测范围 | 0.26-122.8 mg GSH/L |
| 精密度 | 批间差：2.4%批内差：1.8% |
| 检测仪器及波长 | 分光光度计 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，结合本试剂盒的检测范围（0.26-122.8 mgGSH/L），选取最佳稀释倍数，进行正式批量实验。不同样本的稀释比例范围如下表（仅供参考）:
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
人血浆 |
不稀释 |
|
小鼠血清 |
不稀释 |
大鼠血浆 |
不稀释 |
|
10%小鼠脑匀浆 |
不稀释 |
10%胡萝卜匀浆 |
不稀释 |
|
10%大鼠肝匀浆 |
不稀释 |
293T细胞上清 |
不稀释 |
注：稀释液为生理盐水（0.9% NaCl）或PBS（0.01 M，pH 7.4）。
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