Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit
总抗氧化能力(T-AOC)比色法测试盒
| 规格： | / 48T(24 samples) / 96T(48 samples) |
| 价格： | / ￥320 / ￥580 |
货号：E-BC-K136-M
检测仪器及波长: 酶标仪（500-520 nm，最适检测波长520 nm）
样本类型: 血清（浆）;全血;动植物组织;细胞及细胞上清
检测原理
机体中有许多抗氧化物质，能使三价铁离子还原成二价铁离子，后者可与菲啉类物质形成稳固的橙红色络合物，通过比色可测出其抗氧化能力高低。
关键信息
| 指标简称 | T-AOC |
| 样本类型 | 血清（浆）;全血;动植物组织;细胞及细胞上清 |
| 灵敏度 | 0.62 U/mL |
| 检测范围 | 0.62-190.43 U/mL |
| 精密度 | 批间差：5.6%批内差：4.8% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，不同样本稀释比例如下表（仅供参考）：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
人尿液 |
1-2 |
|
10%大鼠肝脏匀浆 |
不稀释 |
HepG2细胞上清 |
不稀释 |
|
10%绿萝组织 |
不稀释 |
HepG2细胞 |
不稀释 |
注：稀释液为生理盐水（0.9% NaCl）或PBS（0.01 M，pH 7.4）。
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