Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Chemical Method)
总抗氧化能力(T-AOC)比色法测试盒(ABTS化学法)
| 规格： | / 48T(32 samples) / 96T(80 samples) |
| 价格： | / ￥200 / ￥320 |
货号：E-BC-K271-M
检测仪器及波长: 酶标仪（730-740 nm）
样本类型: 血清（浆）;尿液;唾液;组织;细胞;植物或中草药抽提液;各种抗氧化物溶液
检测原理
ABTS在适当的氧化剂作用下氧化成绿色的 ，在抗氧化物存在时 的产生会被抑制，在734 nm测定 的吸光度即可测定并计算出样本中的总抗氧化能力。Trolox是一种维生素E的类似物，具有和维生素E相近的抗氧化能力，用作其他抗氧化物总抗氧化能力的参考。例如，Trolox的总抗氧化能力为1，相同浓度情况下，其他物质的总抗氧化能力用其抗氧化能力和Trolox相应的倍数来表示。
关键信息
| 指标简称 | T-AOC |
| 样本类型 | 血清（浆）;尿液;唾液;组织;细胞;植物或中草药抽提液;各种抗氧化物溶液 |
| 灵敏度 | 0.05 mmol/L |
| 检测范围 | 0.05-1.00 mmol/L |
| 精密度 | 批间差：5%批内差：4.1% |
| 检测仪器及波长 | 酶标仪 |
| 自备试剂 | 80%乙醇、1×PBS |
| 保存条件 | -20℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，结合本试剂盒的线性范围（0.05-1.00 mmol/L），选取最佳稀释倍数，进行正式批量实验。不同样本的稀释比例范围如下表（仅供参考）:
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
20%西红柿匀浆 |
2-5 |
人唾液 |
2-5 |
|
10%小鼠心匀浆 |
8-12 |
人尿液 |
15-30 |
|
10%小鼠肝匀浆 |
8-12 |
人血清 |
15-30 |
|
10%小鼠肺匀浆 |
8-12 |
人血浆 |
8-15 |
注：稀释液为1×PBS或80%乙醇。
客户发表文献
- 文献: Treatment of Diabetes Nephropathy in Mice by Germinating Seeds of Euryale ferox through Improving Oxidative Stress
样品类型: Serum - 文献: The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD
样品类型: serum - 文献: Comparison of Selenium Nanoparticles and Sodium Selenite on the Alleviation of Early Atherosclerosis by Inhibiting Endothelial Dysfunction and Inflammation in Apolipoprotein E-Deficient Mice
样品类型: serum - 文献: The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study
样品类型: serum - 文献: Suhuang antitussive capsule ameliorates post-infectious cough in mice through AhR-Nrf2 pathway
样品类型: lung tissue - 文献: Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study
样品类型: saliva - 文献: Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells
样品类型: serum - 文献: Novel Functional Food from an invasive species Polygonum cuspidatum: Safety evaluation, Chemical Composition, and Hepatoprotective Effects
- 文献: Glycine Improved Cryopreserved Spermatozoa Quality in Achai Bull
样品类型: semen - 文献: Ovarian Oxidative Stress Induced Follicle Depletion After Zona Pellucida 3 Vaccination Is Associated With Subfertility in BALB/c Mice
样品类型: Ovarian - 文献: Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway
样品类型: brain tissue - 文献: Preparation of Novel Composites of Polyvinyl Alcohol Containing Hesperidin Loaded ZnO Nanoparticles and Determination of Their Biological and Thermal Properties
- 文献: Flaxseed oil ameliorates aging in D-galactose induced rats via altering gut microbiota and mitigating oxidative damage
样品类型: serum,liver - 文献: Synergistic effect of ZnO nanoparticles and hesperidin on the antibacterial properties of chitosan
样品类型: nanocomposites - 文献: Hematological, Inflammatory, Coagulation, and Oxidative/Antioxidant Biomarkers as Predictors for Severity and Mortality in COVID-19: A Prospective Cohort-Study
样品类型: Serum - 文献: Evaluation of oxidative stress and pro-inflammatory cytokines in occupationally cadmium exposed workers
样品类型: serum - 文献: Spirometric Outcomes and Oxidative Stress Among Cement Factory Workers: Results From a Cross-Sectional Study
样品类型: serum - 文献: Selenium, oxidative stress and inflammatory markers in handicraft workers occupationally exposed to lead
样品类型: serum - 文献: A Nanocurcumin and Pyrroloquinoline Quinone Formulation Prevents Hypobaric Hypoxia-Induced Skeletal Muscle Atrophy by Modulating NF-κB Signaling Pathway
样品类型: gastrocnemius,soleus muscles - 文献: Fabrication of Nutraceutical Beverage from Saffron (Crocus sativus L.) Extract and Studying Its Health Effects
样品类型: serum - 文献: Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin
样品类型: plasma
