Total Superoxide Dismutase (T-SOD) Activity Assay Kit (Hydroxylamine Method)
总超氧化物歧化酶(T-SOD)比色法测试盒(羟胺法)
| 规格： | / 48T(44 samples) / 96T(92 samples) |
| 价格： | / ￥450 / ￥750 |
货号：E-BC-K019-M
检测仪器及波长: 酶标仪（540-560 nm，最佳550 nm）
样本类型: 血清（浆）;尿液;细胞及细胞上清;各种动植物组织
检测原理
通过黄嘌呤及黄嘌呤氧化酶反应系统产生超氧阴离子自由基（O2.-），它氧化羟胺形成亚硝酸盐，在显色剂作用下产生紫红色化合物。当反应体系中加入SOD后，催化超氧阴离子自由基发生歧化反应，使形成的亚硝酸盐减少，进而产生的紫红色变浅。根据样品管与对照管中的吸光度差值，来计算SOD的活性。
关键信息
| 指标简称 | T-SOD |
| 样本类型 | 血清（浆）;尿液;细胞及细胞上清;各种动植物组织 |
| 灵敏度 | 2.4 U/mL |
| 检测范围 | 2.4-61 U/mL |
| 精密度 | 批间差：5.6%批内差：5.5% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | Reagent 4 -20℃，其余2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
2-4 |
人尿液 |
2-5 |
|
大鼠血清 |
4-6 |
10%小鼠肝匀浆 |
160-200 |
|
小鼠血清 |
4-6 |
10%绿萝匀浆 |
3-5 |
|
HepG2细胞匀浆（6.089 mgprot/mL） |
15-30 |
10%小鼠脑匀浆 |
80-100 |
注：稀释液为PBS（0.01 M，pH 7.4）或生理盐水（0.9% NaCl）。
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