Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit
总谷胱甘肽(T-GSH)/氧化型谷胱甘肽(GSSG)比色法测试盒
| 规格： | / 96T(80 samples) |
| 价格： | / ￥580 |
货号：E-BC-K097-M
检测仪器及波长: 酶标仪（405-415 nm，最适检测波长412 nm）
样本类型: 血清（浆）;动物组织;红细胞和培养细胞中的总谷胱甘肽（T-GSH）和氧化型谷胱甘肽（GSSG）
检测原理
通过谷胱甘肽还原酶把GSSG还原成GSH，而GSH可以和生色底物DTNB反应产生GSSG和黄色的TNB，总谷胱甘肽（GSSG+GSH）的量决定了黄色的TNB形成量。从而通过测定A412就可以计算出总谷胱甘肽的量。再用适当试剂先清除样品中的GSH然后利用上述反应原理就可以测定出GSSG的含量。
关键信息
| 指标简称 | T-GSH,GSSG |
| 样本类型 | 血清（浆）;动物组织;红细胞和培养细胞中的总谷胱甘肽（T-GSH）和氧化型谷胱甘肽（GSSG） |
| 灵敏度 | 0.36 μmol/L |
| 检测范围 | 0.36-30 μmol/L |
| 精密度 | 批间差：3.9%批内差：0.6% |
| 检测仪器及波长 | 酶标仪 |
| 自备试剂 | 无水乙醇 |
| 保存条件 | -20℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，不同样本稀释比例如下表（仅供参考）：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
10%大鼠心脏匀浆 |
10-20 |
人血浆 |
不稀释 |
|
10%大鼠肝脏匀浆 |
10-20 |
10%大鼠肾匀浆 |
不稀释 |
|
10%小鼠脑匀浆 |
2-5 |
HepG2细胞 |
不稀释 |
注：稀释液为试剂三。
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